Studies on Chloramphenicol. I

X-ray studies on phospholipid bilayers. XI. Interactions with chloramphenicol.

Chloramphenicol is a widely used antibiotic with low levels of toxicity. However, scanning electron microscopy revealed morphological changes in human erythrocytes when they interacted in vitro with therapeutical concentrations of chloramphenicol. To explain this shape change, a study concerned with the possible interactions of this antibiotic with bilayers built-up of phospholipids located in ...

Pharmacological studies on fatigue. I.

Considerable investigation has been done as to whether or not a particular drug is effective for recovery of an animal after it has become exhausted. Experiments have included screening antifatigue effect on animals, but applications have not been generally established. Effects of drugs on psychological fatigue as seen in man can hardly be inferred from tests on animals. Even physiological effe...

Chloramphenicol.

Chloramphenicol (chloromycetin), an Antibiotic. Pharmacological and Pathological Studies in Animals.

It is a neutral white crystalline substance with a bitter taste and is stable in neutral and acid solutions. The drug is moderately soluble in water (0.25 per cent at room temperature, 0.65 per cent at 700 C and up to 15 per cent in propylene glycol, 5 per cent in 50 per cent acetamide), and is insoluble in vegetable oils. Solubility in urine or serum is about the same as in water. Solutions ar...

Crystallization of type I chloramphenicol acetyltransferase: an approach based on the concept of ionic strength reducers.

Chloramphenicol acetyltransferase (CAT) is responsible for bacterial resistance to chloramphenicol. It catalyzes inactivation of the antibiotic by acetyl-group transfer from acetyl CoA to one or both hydroxyl groups of chloramphenicol. Type I CAT possesses some unique properties which are not observed in other CAT variants. Type I CAT overexpressed in Escherichia coli was purified and crystals ...